The aim of the present study was to investigate the histopathological changes on the kidneys of the brown trout (Salmo trutta m. Fario) inflicted by per os lead poisoning in the 46-week experimental period. The fish were kept in well-oxygenated freshwater basins. Approximately calculated toxic doses of lead-acetate for fish were 550 mg/kg. Following the experimental period, fish organs, in particular kidneys, were examined by light microscopy. Only fish with lesions observed in kidneys were further examined by electron microscopy. An accumulation of proteinaceous material in the glomeruli of the kidneys, and thickening of the basal membrane were observed histopathologically. This proteinaceous material was also observed in the lumina of the kidney tubules. Electron microscopic examination completely supported this finding. Furthermore, to the authors' previous knowledge, undocumented damage of the brush borders of the proximal kidney tubules was also observed.

Kvalitet i bezbednost upotrebe biljnih preparata ostaju i dalje prioritet koji treba zakonski urediti na svetskom nivou. Za registraciju biljnog preparata kao leka postoje isti zahtevi kao i za registraciju drugih lekova u pogledu dokumentacije koja treba da sadrži predklinicka i klinicka ispitivanja. Ovakva ispitivanja vrlo su zahtevna, a kako složenost molekulske građe, cak i jednokomponentnih biljnih preparata, ometa utvrđivanje jedinstvenih kriterijuma za jasno pracenje njihovih terapijskih efekata, ispunjavanje zahteva za registrovanje biljnih preparata kao lekova u praksi jos uvek je izazov i za naucnike i za proizvođace.