The aims of this study are the isolation and identification of possible bacteriological agents in respiratory infections of calves and the optimization of a diagnostic protocol to identify Arcanobacterium haemolyticum. Lesions of lungs from calves with pneumonia were examined. Cultural, morphological and conventional biochemical testing were done. The investigation was complemented by the double CAMP test. Five strains of Arcanobacterium haemolyticum in pure culture were found. The presence of Arcanobacterium haemolyticum in the lungs of calves with pneumonia was established and, consequently, more attention should be paid to this species in everyday laboratory work. The cultural similarity of Arcanobacterium haemolyticum to common bacteria like beta-hemolytic Streptococcus spp. and Arcanobacterium pyogenes is probably responsible for rare reports on the isolation of Arcanobacterium haemolyticum in veterinary microbiology. Our results indicate that Arcanobacterium haemolyticum could be or is the etiological agent of pneumonia. Therefore, we suggest the diagnostic protocol available for routine work in most microbiological laboratories. [Projekat Ministarstva nauke Republike Srbije, br. 41012]

Phenazines, secondary metabolites of fluorescent Pseudomonas, represent a group of heterocyclic nitrogen-containing compounds showing a broad spectrum of antibiotic properties. Phenazines producing fluorescent Pseudomonas species are studied extensively for their application in plant disease management. In this study, we examined the antifungal activity of different indigenous Pseudomonas isolates (Q16, B25 and PS2) against the phytopathogenic fungus Alternaria tenuissima, which had infected cardoon (Cynara cardunculus L., Asteraceae). An in vitro experiment demonstrated the antifungal activity of selected indigenous isolates. In addition, an in vivo experiment under gnotobiotic conditions showed suppression of C. cardunculus disease caused by A. tenuissima. The quantification of phenazines revealed significant amounts of phenazine-1-carboxylic acid (PCA) and 2-hydroxy-phenazine-1-carboxylic acid (2-OH-PCA). PCR analysis confirmed the presence of PCA genes in all examined indigenous Pseudomonas isolates. Based on our results, we assume that these Pseudomonas isolates have potential in controlling plant diseases caused by A. tenuissima. [Projekat Ministarstva nauke Republike Srbije, br. III46007 and br. TR31018]

This work presents a methodological approach to the identification of aspects and evaluation the environmental impact, in order to optimize the model of risk assessment of environmental pollution for the mining projects, in order to improve and advance this process on the example of the project 'Magnesite Mining in the Deposit Cavlovac - Masnica' in the area of the municipality Cajetina. .