Remote control is one of the best solutions for managing inaccessible systems. Many researches have been made in the field of the remote control [1-4]. These researches have improved certain aspects of industry, medicine, military, etc. The benefits of remote control are numerous such as: operating in hazard environment, telemedicine, missile guidance, etc. The most important characteristic of remote control is operating in real-time as shown in papers form references [5-7].

Modern breeds of dairy animals are able to produce huge amount of milk. In attempt to consume, digest and metabolize enough nutrients to satisfy lactation needs, those animals are exposed to serious stress conditions that can affect their health. Health problems which arise from those conditions are mainly related to impaired ability to metabolize enough nutrients to compensate for those lost in milk. They are known as metabolic or production diseases and may be of great economic importance in milk production systems.

Angiogenesis is a critical event in the development, progression, and spread of various human cancers, including lung cancer. Molecular mechanisms that underlie the complex regulation of angiogenic processes are poorly understood. However, an increasing body of evidence indicates miRNAs as important regulators of tumor angiogenesis. Forceps biopsies were collected from tumor tissue, surrounding tissue, and non-tumor tissue from 50 NSCLC patients. Lung tissue samples from individuals with no clinical evidence of a cancerous disease served as controls. Immunohistochemical staining for Factor VIII was used to evaluate microvessel density (MVD). TaqMan® primer-probe sets were used in quantitative real-time RT-PCR reactions to determine expression levels of let-7b, miR-126, miR-9, and miR-19a. We demonstrated significantly higher MVD and decreased expression levels of let-7b and miR-126 in tumor tissue and surrounding tissue in comparison to corresponding non-tumor tissue or lung tissue from the control group. In addition, no differences in MVD and expression levels of both miRNAs between tumor tissue and surrounding tissue from NSCLC patients were observed. Low expression of both miRNAs correlated with high MVD and worse progression-free survival and overall survival. These observations strongly suggest similar molecular alternations within tumor tissue and surrounding tissue that comprise a specific microenvironment. Low expression of let-7b and miR-126 seems to have a possible anti-angiogenic role in lung tumor tissue and significantly correlates with worse survival outcomes for lung cancer patients. Moreover, the regulation of let-7b and miR-126 expression could have therapeutic potential because it could reduce tumor angiogenesis and therefore suppress tumor growth in lung cancer patients.

U prvom delu rada, za karakteristican primer složene hemijske reakcije, proanalizirano je nekoliko matematickih metoda za resavanje sistema diferencijalnih jednacina kojima se opisuje proces, a koje se najcesce koriste u inženjerskoj teoriji i praksi. Analiza je izvrsena sa aspekta neophodnog matematickog znanja, potrebnog vremena za resavanje problema, mogucnosti greske kao i kontrole dobijenih rezultata. U drugom delu rada, dat je predlog za efikasnije resavanje posmatranog problema pri cemu je primenjen princip redukcije sistema na jednu diferencijalnu jednacinu, koju je najpogodnije posmatrati kao nehomogenu jednacinu I reda sa konstantnim koeficijentima, zavisnu od vremena. Pokazano je da je predložena metoda pogodnija u odnosu na postojece, s obzirom da ima određene prednosti i može se koristiti kao alternativa istim.

Background The polarized reorganization of the T cell membrane and intracellular signaling molecules in response to T cell receptor (TCR) engagement has been implicated in the modulation of T cell development and effector responses. In siRNA-based studies Dlg1, a MAGUK scaffold protein and member of the Scribble polarity complex, has been shown to play a role in T cell polarity and TCR signal specificity, however the role of Dlg1 in T cell development and function in vivo remains unclear. Methodology/Principal Findings Here we present the combined data from three independently-derived dlg1-knockout mouse models; two germline deficient knockouts and one conditional knockout. While defects were not observed in T cell development, TCR-induced early phospho-signaling, actin-mediated events, or proliferation in any of the models, the acute knockdown of Dlg1 in Jurkat T cells diminished accumulation of actin at the IS. Further, while Th1-type cytokine production appeared unaffected in T cells derived from mice with a dlg1germline-deficiency, altered production of TCR-dependent Th1 and Th2-type cytokines was observed in T cells derived from mice with a conditional loss of dlg1 expression and T cells with acute Dlg1 suppression, suggesting a differential requirement for Dlg1 activity in signaling events leading to Th1 versus Th2 cytokine induction. The observed inconsistencies between these and other knockout models and siRNA strategies suggest that 1) compensatory upregulation of alternate gene(s) may be masking a role for dlg1 in controlling TCR-mediated events in dlg1 deficient mice and 2) the developmental stage during which dlg1 ablation begins may control the degree to which compensatory events occur. Conclusions/Significance These findings provide a potential explanation for the discrepancies observed in various studies using different dlg1-deficient T cell models and underscore the importance of acute dlg1 ablation to avoid the upregulation of compensatory mechanisms for future functional studies of the Dlg1 protein.