Introduction: Dehydration develops when the body fluid losses exceed fluid intake. It may occur during exercise, heat stress, restricted fluid intake, or any combination of these. Marginal dehydration (loss of > 2% body weight) can compromise aerobic exercise performance, particularly in hot weather conditions, and may disturb fluid and electrolyte balance. The aim of the study was to determine the quantity, type and dynamic of fluid intake during athletic performance in endurance sports (football and basketball) in two age categories: juniors (under the age of 18) and seniors (over the age of 18).Methods: Research included 100 athletes playing in Premier League in Bosnia-Herzegovina. We formed groups by sport type (football and basketball) and age (<18 and ≥18 years). Questionnaire with questions about the fluid intake habits was used for data collection.    Results: There were 53 football players and 47 basketball players. All the participants were male. Average age of the participants was 19.3 ± 4.58. Habit of weighing before and after training was present in less than 44% of players among all the groups. Seniors were more frequently measuring their weight compared to junior players (p=0.01). Basketball players and players younger than 18 years were most frequently taking more than 2L of water per day. Most of the players, regardless of sport type or age group were not taking at least ½ L of isotonic fluid before the training. Signs of dehydration were more frequently observed in players under 18 years old, with most frequent sign being dry throat and sudden fatigue.Conclusion: Water and electrolytes intake before, during and after training of the athletes were inadequate regardless of type of sports and the age of athletes.

Introduction: Glycated hemoglobin (HbA1c) is formed by non-enzymatic binding of glucose to the free amino group of the N-terminal end of the s-chain of hemoglobin A. HbA1c is representative of the mean blood glucose level over three months. The aim of the study was to evaluate the Hemoglobin A1c immunoturbidimetric assay performance on two different commercial systems.Methods: We evaluated the precision and trueness for determination of HbA1c in whole blood. Concentrations of total hemoglobin and HbA1c were evaluated on Dimension Xpand (Siemens) and Cobas 501 (Roche) analyzers. HbA1c was measured in a latex agglutination inhibition test. Commercial controls Liquichek Diabetes Control Level 1 and Liquichek Diabetes Control Level 2 (Bio Rad) at two levels were used for quality control. Analytical validation of HbA1c included: within-run imprecision, between-day imprecision, inaccuracy and comparison determination on the human samples on 2 systems: Dimension Xpand and Cobas 501 analyzers. Results: Within-run imprecision on the commercially controls for Level 1 is 4.5% and Level 2 is 3.2% between-day imprecision on commercially controls is 6.1% Level 1 and 5.1% Level 2 for respectively inac- curacy on commercially controls for Level 1 is 1.8% and Level 2 is 4.8%. Method comparison on human samples shows the correlation coefficient of 0.99.Conclusion: The presented results of the analytical evaluation methods for the determination of HbA1c showed an acceptable accuracy and precision.

We analyze the bounds on the Higgs pseudo-observables following from electroweak constraints, under the assumption that the Higgs particle is the massive excitation of an $SU(2)_L$ doublet. Using such bounds, detailed predictions for $h\to 4\ell$ decay rates, dilepton spectra, and lepton-universality ratios are presented.