This study aimed to investigate the concentrations of cholesterol and triglycerides in the serum of sheep from the area of Livanjsko Polje and Mount Vlasic. A total of 117 sheep of the breed pramenka were included in the research. The sheep were divided into groups according to the sampling area through different periods (July, August, and September). Using an automatic analyzer (Kodak Ektachem analyzer DT 60 II, DTSC Module, and DTE Module), the concentrations of cholesterol and triglycerides were determined in the serum of sheep. In the area of Livanjsko Polje, the highest established value of cholesterol in sheep serum was in the second sampling period, with statistical significance compared to the other two sampling periods. Cholesterol values in the Vlasic Mountain area were within the physiological range. The highest established triglyceride concentration in the area of Livanjsko Polje was also the second sampling period, with statistical significance (p <0.05) compared to other sampling periods, as well as the same period from the Travnik area. The results of triglycerides in the blood serum of sheep from the Vlasic mountain area show a tendency to decrease in the third sampling period, at the end of the lactation period. These results may be due to the effects of various factors, such as age, gender, health, lactation status, and sheep breed.

We have performed diagnostics of sub clinical mastitis in three different cow breeds with comparison of California mastitis test results, somatic cells count at quarter level and with bacteriological findings confirmation in order to justify their appliance in mastitis diagnostics. In total, 90 cows or 360 quarters of mammary gland have been examined. In 63.3 % of the examined cows, with different racial origin, positive reaction to California mastitis test have been established. Usually, positive reaction have been found in milk of one and two quarters, with reaction intensity of one and two plus. SSC higher than 200.000 in 1 ml is established in 60 % of cases and the most often causes of mastitis were staphylococcae, streptococcae and mixed infections. With continuous use of this methods it is possible to timely detect presence of sub clinical mastitis and so to obtain satisfactory results in prevention and therapy of mastitis, as well as improvement in amount and quality of milk.