s from the Food Allergy and Anaphylaxis Meeting 2016 Rome, Italy. 13–15 October 2016 © The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. ORAL ABSTRACT SESSION 1: Food allergens • Anaphylaxis OP01 Fatal anaphylaxis is decreasing in France: analysis of national data, 1979–2011 Guillaume Pouessel, Claire Claverie, Julien Labreuche, Jean‐Marie Renaudin, Aimée Dorkenoo, Mireille Eb, Anne Moneret‐Vautrin, Antoine Deschildre, Stephane Leteurtre Department of Pediatrics, Children’s Hospital, Roubaix, France; Division of Pulmonology and Allergology, Department of Pediatrics, Faculty of Medicine and Children’s Hospital, Lille, France; Allergy Vigilance Network, Vandoeuvre les Nancy, France; Université Lille 2, CHU Lille, EA 2694 ‐ Santé Publique: épidémiologie et qualité des soins, Lille, France; Biostatistic Unit, Maison Régionale de la Recherche Clinique, CHRU Lille, Lille, France; Department of Allergology, Emile Durkheim Hospital, Epi‐ nal, France; Centre d’Epidémiologie sur les Causes Médicales de Décès INSERM, CHU de Bicêtre, Le Kremlin‐Bicêtre, France Correspondence: Guillaume Pouessel ‐ guillaume.pouessel@gmail.com Clinical and Translational Allergy 2017, 7(Suppl 1):OP0

In murine systemic lupus erythematosus (SLE), aberrant regulation of interferon (IFN)-alpha-STAT1 signaling and perturbed homeostasis of CD4+FOXP3+ regulatory T cells (Tregs) were described. In the present study, STAT1 signaling and circulating Treg subsets were assessed by flow cytometry in 39 SLE patients and their potential association with disease course was examined during long-term follow-up. Levels of STAT1 protein as measured by median fluorescence intensity (MFI) were significantly increased in SLE CD4 T cells when compared with rheumatoid arthritis patients and healthy controls and were positively correlated with disease activity. The highest STAT1 MFI was found in CD45RA-FOXP3hi-activated Treg (aTreg) subset, which demonstrated the highest STAT1 phosphorylation responses among SLE CD4 T cells and significant decrease in proliferation marker Ki-67 expression after IFN-alpha stimulation. Percentage of Ki-67+ aTregs was significantly decreased in SLE patients and was negatively correlated with CD4 T cell STAT1 MFI. A subgroup of SLE patients characterized by lower aTreg counts experienced more severe relapsing disease course during 1,000 days of follow-up. Mean CD4 T cell STAT1 MFI in follow-up samples from SLE patients was negatively correlated with mean of follow-up aTreg counts. Our findings indicate that augmented STAT1 signaling may be involved in perturbed aTreg homeostasis, which could represent a possible marker of SLE disease severity.

Table of contentsWORKSHOP 4: Challenging clinical scenarios (CS01–CS06)CS01 Bullous lesions in two children: solitary mastocytomaS. Tolga Yavuz, Ozan Koc, Ali Gungor, Faysal GokCS02 Multi-System Allergy (MSA) of cystic fibrosis: our institutional experienceJessica Hawley, Christopher O’Brien, Matthew Thomas, Malcolm Brodlie, Louise MichaelisCS03 Cold urticaria in pediatric age: an invisible cause for severe reactionsInês Mota, Ângela Gaspar, Susana Piedade, Graça Sampaio, José Geraldo Dias, Miguel Paiva, Mário Morais-AlmeidaCS04 Angioedema with C1 inhibitor deficiency in a girl: a challenge diagnosisCristina Madureira, Tânia Lopes, Susana Lopes, Filipa Almeida, Alexandra Sequeira, Fernanda Carvalho, José OliveiraCS05 A child with unusual multiple organ allergy disease: what is the primer?Fabienne Gay-CrosierCS06 A case of uncontrolled asthma in a 6-year-old patientIoana-Valentina Nenciu, Andreia Florina Nita, Alexandru Ulmeanu, Dumitru Oraseanu, Carmen ZapucioiuORAL ABSTRACT SESSION 1: Food allergy (OP01–OP06)OP01 Food protein-induced enterocolitis syndrome: oral food challenge outcomes for tolerance evaluation in a Pediatric HospitalAdrianna Machinena, Olga Domínguez Sánchez, Montserrat Alvaro Lozano, Rosa Jimenez Feijoo, Jaime Lozano Blasco, Mònica Piquer Gibert, Mª Teresa Giner Muñoz, Marcia Dias da Costa, Ana Maria Plaza MartínOP02 Characteristics of infants with food protein-induced enterocolitis syndrome and allergic proctocolitisEbru Arik Yilmaz, Özlem Cavkaytar, Betul Buyuktiryaki, Ozge Soyer, Cansin SackesenOP03 The clinical and immunological outcomes after consumption of baked egg by 1–5 year old egg allergic children: results of a randomised controlled trialMerrynNetting, Adaweyah El-Merhibi, Michael Gold, PatrickQuinn, IrmeliPenttila, Maria MakridesOP04 Oral immunotherapy for treatment of egg allergy using low allergenic, hydrolysed eggStavroula Giavi, Antonella Muraro, Roger Lauener, Annick Mercenier, Eugen Bersuch, Isabella M. Montagner, Maria Passioti, Nicolò Celegato, Selina Summermatter, Sophie Nutten, Tristan Bourdeau, Yvonne M. Vissers, Nikolaos G. PapadopoulosOP05 Chemical modification of a peanut extract results in an increased safety profile while maintaining efficacyHanneke van der Kleij, Hans Warmenhoven, Ronald van Ree, Raymond Pieters, Dirk Jan Opstelten, Hans van Schijndel, Joost SmitOP06 Administration of the yellow fever vaccine in egg allergic childrenRoisin Fitzsimons, Victoria Timms, George Du ToitORAL ABSTRACT SESSION 2: Asthma (OP07–OP12)OP07 Previous exacerbation is the most important risk factor for future exacerbations in school-age children with asthmaS. Tolga Yavuz, Guven Kaya, Mustafa Gulec, Mehmet Saldir, Osman Sener, Faysal GokOP08 Comparative study of degree of severity and laboratory changes between asthmatic children using different acupuncture modalitiesNagwa Hassan, Hala Shaaban, Hazem El-Hariri, Ahmed Kamel Inas E. MahfouzOP09 The concentration of exhaled carbon monoxide in asthmatic children with different controlled stadiumPapp Gabor, Biro Gabor, Kovacs CsabaOP10 Effect of vitamin D3 supplementation during pregnancy on risk of persistent wheeze in the offspring: a randomised clinical trialBo Chawes, Klaus Bønnelykke, Jakob Stokholm, Lene Heickendorff, Susanne Brix, Morten Rasmussen, Hans BisgaardOP11 Lung function development in childhoodHenrik Wegener Hallas, Bo Chawes, Lambang Arianto, Hans BisgaardOP12 Is the effect of maternal and paternal asthma different in female and male children before puberty?Maike Pincus, Thomas Keil, Andreas Reich, Ulrich Wahn, Susanne Lau, Linus GrabenhenrichORAL ABSTRACT SESSION 3: Epidemiology—genetics (OP13–OP18)OP13 Lifestyle is associated with incidence and category of allergen sensitisation: the ALADDIN birth cohortSara Fagerstedt, Helena Marell Hesla, Emelie Johansson, Helen Rosenlund, Axel Mie, Annika Scheynius, Johan AlmOP15 Maternal filaggrin mutations increase the risk of atopic dermatitis in children: an effect independent of mutation inheritanceJorge Esparza-Gordillo, Anja Matanovic, Ingo Marenholz, Anja Bauerfeind, Klaus Rohde, Katja Nemat, Min-Ae Lee-Kirsch, Magnus Nordenskjöld, Marten C. G. Winge, Thomas Keil, Renate Krüger, Susanne Lau, Kirsten Beyer, Birgit Kalb, Bodo Niggemann, Norbert Hübner, Heather J. Cordell, Maria Bradley, Young-Ae LeeOP16 Allergic multimorbidity of asthma, rhinitis and eczema in the first 2 decades of the German MAS birth cohortThomas Keil, Hannah Gough, Linus Grabenhenrich, Dirk Schramm, Andreas Reich, John Beschorner, Antje Schuster, Carl-Peter Bauer, Johannes Forster, Fred Zepp, Young-Ae Lee, Renate Bergmann, Karl Bergmann, Ulrich Wahn, Susanne LauOP17 Childhood anaphylaxis: a growing concernFilipe Benito Garcia, Inês Mota, Susana Piedade, Ângela Gaspar, Natacha Santos, Helena Pité, Mário Morais-AlmeidaOP18 Indoor exposure to molds and dampness in infancy and its association to persistent atopic dermatitis in school age. Results from the Greek ISAAC II studyAthina Papadopoulou, Despina Mermiri, Elpida Xatziagorou, Ioannis Tsanakas, Stavroula Lampidi, Kostas PriftisORAL ABSTRACT SESSION 4: Pediatric rhinitis—immunotherapy (OP19–OP24)OP19 Associations between residential greenness and childhood allergic rhinitis and aeroallergen sensitisation in seven birth cohortsElaine Fuertes, Iana Markevych, Gayan Bowatte, Olena Gruzieva, Ulrike Gehring, Allan Becker, Dietrich Berdel, Michael Brauer, Chris Carlsten, Barbara Hoffmann, Anita Kozyrskyj, Caroline Lodge, Göran Pershagen, Alet Wijga, Heinrich JoachimOP20 Full symptom control in pediatric patients with allergic rhinitis and asthma: results of a 2-year sublingual allergen immunotherapy studyZorica Zivkovic, Ivana Djuric-Filipovic, Jasmina Jocić-Stevanovic, Snežana ZivanovicOP21 Nasal epithelium of different ages of atopic subjects present increased levels of oxidative stress and increased cell cytotoxicity upon rhinovirus infectionStyliani Taka, Dimitra Kokkinou, Aliki Papakonstantinou, Panagiota Stefanopoulou, Anastasia Georgountzou, Paraskevi Maggina, Sofia Stamataki, Vassiliki Papaevanggelou, Evangelos Andreakos, Nikolaos G. PapadopoulosOP22 Cluster subcutaneous immunotherapy schedule: tolerability profile in childrenMonica Piquer Gibert, Montserrat Alvaro Lozano, Jaime Lozano Blasco, Olga Domínguez Sánchez, Rosa Jiménez Feijoo, Marcia Dias da Costa, Mª Teresa Giner Muñoz, Adriana Machinena Spera, Ana Maria Plaza MartínOP23 Rhinitis as a risk factor for asthma severity in 11-year old children: population-based cohort studyMatea Deliu, Danielle Belgrave, Angela Simpson, Adnan CustovicOP24 The Global Lung Function Initiative equations in airway obstruction evaluation of asthmatic childrenJoão Gaspar Marques, Pedro Carreiro-Martins, Joana Belo, Sara Serranho, Isabel Peralta, Nuno Neuparth, Paula Leiria-PintoPOSTER DISCUSSION SESSION 1: Food allergy (PD01–PD05)PD01 Allergen-specific humoral and cellular responses in children who fail egg oral immunotherapy due to allergic reactionsMarta Vazquez-Ortiz, Mariona Pascal, Ana Maria Plaza, Manel JuanPD02 FoxP3 epigenetic features in children with cow milk allergyLorella Paparo, Rita Nocerino, Rosita Aitoro, Ilaria Langella, Antonio Amoroso, Alessia Amoroso, Carmen Di Scala, Roberto Berni CananiPD04 Combined milk and egg allergy in early childhood: let them eat cake?Santanu Maity, Giuseppina Rotiroti, Minal GandhiPD05 Introduction of complementary foods in relation to allergy and gut microbiota in farm and non-farm childrenKarin Jonsson, Annika Ljung, Bill Hesselmar, Ingegerd Adlerbert, Hilde Brekke, Susanne Johansen, Agnes Wold, Ann-Sofie SandbergPOSTER DISCUSSION SESSION 2: Asthma and wheeze (PD06–PD16)PD06 The association between asthma and exhaled nitric oxide is influenced by genetics and sensitisationBjörn Nordlund, Cecilia Lundholm, Villhelmina Ullemar, Marianne van Hage, Anne Örtqvist, Catarina AlmqvistPD09 Prevalence patterns of infant wheeze across EuropeAnna Selby, Kate Grimshaw, Thomas Keil, Linus Grabenhenrich, Michael Clausen, Ruta Dubakiene, Alessandro Fiocchi, Marek Kowalski, Nikos Papadopoulos, Marta Reche, Sigurveig Sigurdardottir, Aline Sprikkleman, Paraskevi Xepapadaki, Clare Mills, Kirsten Beyer, Graham RobertsPD10 Epidemiologic changes in recurrent wheezing infantsHerberto Jose Chong Neto, Gustavo Falbo Wandalsen, Ana Carolina Dela Bianca, Carolina Aranda, Nelson Augusto Rosário, Dirceu Solé, Javier Mallol, Luis García MarcosPD13 A single nucleotide polymorphism in the GLCCI1 gene is associated with response to asthma treatment in childrenIvanaBanic, Matija Rijavec, Davor Plavec, Peter Korosec, Mirjana TurkaljPD14 Pollen induced asthma: Could small molecules in pollen exacerbate the protein-mediated allergic response?Alen Bozicevic, Maria De Mieri, Matthias HamburgerPD15 A qualitative study to understand how we can empower teenagers to better self-manage their asthmaSimone Holley, Ruth Morris, Frances Mitchell, Rebecca Knibb, Susan Latter, Christina Liossi, Graham RobertsPD16 Polymorphism of endothelial nitric oxide synthase (eNOS) gene among Egyptian children with bronchial asthmaMostafa M. M. HassanPOSTER DISCUSSION SESSION 3: Mechanisms—Epidemiology (PD17–PD21)PD17 Pregnancy outcomes in relation to development of allergy in a Swedish birth cohortMalin Barman, Anna Sandin, Agnes Wold, Ann-Sofie SandbergPD18 Evolution of the IgE response to house dust mite molecules in childhoodDaniela Posa, Serena Perna, Carl-Peter Bauer, Ute Hoffmann, Johannes Forster, Fred Zepp, Antje Schuster, Ulrich Wahn, Thomas Keil, Susanne Lau, Kuan-Wei Chen, Yvonne Resch, Susanne Vrtala, Rudolf Valenta, Paolo Maria MatricardiPD19 Antibody recognition of nsLTP-molecules as antigens but not as allergens in the German-MAS birth cohortOlympia Tsilochristou, Alexander Rohrbach, Antonio Cappella, Stephanie Hofmaier, Laura Hatzler, Carl-Peter Bauer, Ute Hoffmann, Johannes Forster, Fred Zepp, Antje Schuster, RaffaeleD’Amelio, Ulrich Wahn, Thomas Keil, Susanne Lau, Paolo Maria MatricardiPD20 Early life coloniza