Introduction: The technique of closing the appendiceal stump using linear titanium stapler clips is being used more and more frequently in laparoscopic surgery, despite the good practice in the use of resorbable single endoloop vicryl ligatures and/or the non-resorbable plastic hem-o-lok clips. No light has been shed on potential undesirable effects on the haemathological profile of the use of titanium stapler clips. This experimental study aimed at establishing any possible undesirable effect of linear titanium stapler clips and single resorbable vicryl endoloop ligatures on the blood cells in rats following appendectomy. Methods: A total of 70 adult rats were used, divided into a control group (n = 10), and two experimental groups (n = 30 + 30). The appendices were removed from the rats in the first experimental group using titanium stapler clips and in the second experimental group the appendiceal stump using resorbable endoloop vicryl ligatures was closed. In both experimental groups three sub-groups with ten animals each were formed, from which peripheral blood from a tail vein on days 7, 28 and 60 was removed postoperatively, in order to assess the usual haematological parameters. Stained blood smears were also analysed in order to establish any poikilocytotic erythrocytes present. Results: In the first experimental group, with the titanium stapler clips, more than 25 % neutrophils were found on day 7, which is a significantly different result (p < 0.05) to the control group. In the second experimental group, there were more neutrophils than in the titanium stapler clips group, especially on days 7 and 60 and the results of these two sub-groups differ statistically significantly, p < 0.05. Hypochromia was found in the endoloop vicryl ligatures group, as well as in the titanium stapler clips sub-group on day 28, due to lower haemoglobin values which were significantly different to the control group, p < 0.05. Moderate levels of annulocytes, spherocytes and stomatocytes were found in most experimental groups. Conclusion: The results of this study favour the use of linear titanium stapler clips over resorbable single endoloop vicryl ligatures, because a less unfavourable effect was established on the blood cells of the experimental rats with their use.

Background and Objectives: Titanium DS clips are made to secure the appendiceal stump during laparoscopic appendectomy. The assumption is that they behave like stapler clips in the body, being made from titanium. However, the construction and weight of DS clips differs from staplers. Their biocompatibility may have implications for their clinical use. Methods: One hundred and twenty rats were randomized into four experimental groups: in the first group the appendiceal stump was secured by Vicryl® ligature, in the second by linear staplers, in the third by Hem-o-lok® plastic clips, and in the fourth by DS titanium clips. Ten animals from each group were sacrificed 7, 28, and 60 days post-surgery. Histopathological data and adhesion formation were assessed. Results: On postoperative day 7, a statistically significant difference was found in the occurrence of inflammation between the Hem-o-lok® and stapler groups. The adhesion score was significantly higher in the Vycril® and Hem-o-lok® in comparison to the DS and stapler groups. On postoperative day 28, a statistically significant difference was found in the occurrence of inflammation between the Vycril® and stapler groups. The adhesion score was significantly higher in the Vycril® than in the DS and stapler groups. On postoperative day 60, there were no statistical differences between any of the groups. The adhesion score was significantly higher in the Hem-o-lok® group in comparison to the DS and stapler groups. Conclusion: Staplers and DS clips have advantages over other methods of securing the appendiceal stump, because of their high biocompatibility and lower adhesion score.

The study of crystalluria is of great importance for the detection of substances of endogenous or exogenous origin that are present inthe urine, to a greater or lesser extent. Urinary sediment crystals can provide valuable answers for the assessment of therapeutic efficacy, as well as congenital and/or acquired pathophysiological conditions. The nature of the observed crystals informs the clinician of the biochemical irregularity of the urine. Crystalluria is of clinical significance only if it has been studied under good test conditions (sample selection, time and storage conditions). Crystalluria interpretations are performed on the basis of the urinary pH determined with statistically significant reliability. When studying crystalluria by light microscopy, it is necessary to provide light polarization or bidirectional illuminationin order to reduce the risk of diagnostic error.

The coexistence of humans and dogs, in addition to all positive effects, can result in negative effects on human health. A particular risk is posed by a population of stray dogs, that is, dogs without owners and veterinary supervision. A contact with dogs in addition to bites, carries the risk of viral, bacterial and parasitic zoonoses, and can also cause psychological trauma. Children, the elderly and pregnant women are the categories most susceptible to the negative effects of dogs. The aim of the paper was to make an interdisciplinary analysis of the negative effects of dogs on humans. Dog bites cause wounds and dysfunction of damaged tissue, and often lead to various infections. The risks of rabies and tetanus are particularly significant if proper and timely treatment is not performed. Ongoing training for dog owners can significantly reduce the number of bites inflicted by owned dogs, but stray dogs remain a serious social problem and pose potential health risks of some zoonosis. Timely and adequate management of bite wounds and the use of rabies-post-exposure prophylaxis as well as psycho-therapy, where indicated, significantly reduce possible adverse health effects for patients who have been bitten by dogs.

Derived from mesenchymal stem cells (MSCs) under the influence of various local factors and growth hormones, osteoblasts are one of the leading components of the bone tissue. 1,25-dihydroxyvitamin D3 (calcitriol), an active form of vitamin D, plays an important role in retaining calcium and phosphate homeostasis within the body. Since vitamin D is also known to have effects on cell proliferation, our study aimed to investigate the effects of different concentrations of calcitriol on the proliferation of in vitro cultured osteoblast-like cells from the bone marrow of 90-95 days old young adult rats of both sexes. Calcitriol was applied at six (6) different concentrations (nM): 0.1, 1, 10, 20, 40 i 60. Each concentration was tested four times, counting the cells after 24 and 48 hours. After 24 and 48 hours treatment, the most effective dose of calcitriol for cell proliferation was 0.1 nM for both males and females. The greatest reduction in the frequency of OB-like cells from both sexes after treatment of 24 and 48 hours, was a 60 nM calcitriol concentration. Higher proliferative values of osteoblast-like cells were obtained in males compared to those obtained in females. Thus, the results of the current study reveal that calcitriol treatment induces the proliferation of osteoblast-like cells in a dose-dependent manner and this effect shows difference in cells from different genders.

Ksanten turevleri, dogal, yari sentetik ve sentetik kokenli olabilen siklik, organik bilesikleri temsil eder. Bu bilesikler, antibakteriyel, antiviral ve antienflamatuar ozellikler gibi cesitli farmakolojik aktiviteler nedeniyle dikkat cekmektedirler. Ayrica, ilaca direncli losemi hatlari icin ve fotodinamik tedavide antagonistler olarak kullanilmaktadirlar. Ayrica lazer teknolojisinde boyalar ve biyomolekullerin gorsellestirilmesi icin pH'a duyarli floresan malzemeler olarak uygulanirlar. Veteriner hekimlik alaninda yeni hastaliklar nedeniyle, bu bilesiklerin uygulanmasi cok faydali olabilir ve literaturde Veteriner arastirma alanindaki benzer bilesiklerin aktivitesi aciklanmaktadir. Bu derlemede ksanten turevlerinin antibakteriyel, antifungal, antihelmintik, antiprotozoal, antikanserojenik ve antidiyabetik aktivite gibi en onemli aktiviteleri ve etki mekanizmalari sunulmustur.

Cytological analysis of urine sediment is an integral part of urine analysis and a useful, cost-effective diagnostic tool for all routine clinical examinations, which can sometimes be overlooked in veterinary practice. Proper handling and timely urine sample analysis are essential for valid microscopic analysis. Microscopic urinary sediment testing is usually carried out with stained or unstained specimen. A common urine may contain a small number of cells (erythrocytes and leukocytes, epithelial cells) of several crystals, spermatozoids (male animals). In contrast, a large number of cells or casts, the presence of unusual types of crystals, neoplastic cells, parasites, and microorganisms include abnormal findings, requiring a specialized diagnostic approach. Correct identification of organic components of the urinary sediment are crucial for accurate and timely diagnosis of kidney disorders. Laboratory experts should be trained to properly identify and classify organic components of the urinary tract.

Bites of laboratory animals are treated as the bites of any other animals since the possibility of existence of pathogenic microorganisms, unfavorable for human health, in the rooms where these animals reside, is not excluded. A rare case of a laboratory rat bite, used for scientific research and previously infected with Pseudomonas aeruginosa , is presented here. The patient's wound was located on the forefinger of his hand and was 1 cm long and up to 0.2 cm deep. The antitetanus prophylaxis was administered in an ambulance, including antibiotic therapy with amoxicillin-clavulanate per os. There exists a need for checks and special guidelines for the handling and retention of laboratory animals. The patient has successfully remedied the wound, thanks to consistent antibiotic therapy and antitetanus prophylaxis, and possible inflammatory complications were prevented .

Post-traumatic osteomyelitis (OM) is an uncommon event in cats, usually affecting distal phalanges of extremities. Tail injuries seldom cause bone infection, but often result in neural damage with subsequent tail paralysis, and occasionally in urinary/fecal incontinence. We present a case of old stray cat which developed post-traumatic tail OM, and endured it for years. It was an immuno-compromised, neglected, animal strongly infested with larvae of Aelurostrongylus abs trusus and oocistae of Isospora felis. Ultimately, it was treated by tail amputation, with without any health consequences.  Relevance and novel information: This report describes the management and outcome of a rare and a life-threatening case of feline post-traumatic  tail OM which was previously not reported in literature.