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29. 12. 2009.
Determination of Coenzyme A (CoASH) in the Presence of Different Thiols by Using Flow-Injection with a UV/Vis Spectrophotometric Detector and Potentiometric Determination of CoASH Using an Iodide ISE
Coenzyme A (CoA or CoASH) is one of the most important biologically active compounds, and for this reason a reliable, fast and simple determination of this species is needed. We describe a simple and fast assay of CoASH using potentiometric flow-injection analysis and spectrophotometric kinetic determination. The described methods are suitable for use over a wide CoASH concentration range (1×10-6 – 1×10-4 M).